Pesticides are used to protect crops and increase crop yield by killing or mitigating harmful insects and small animals. However, the increased usage of pesticides has adverse effects on human health and the environment. Therefore, accurate monitoring and analysis of pesticide concentration in the environment is essential. The Institute of Environmental Science and Research Limited and the Ministry for Primary Industries have coordinated different surveys to monitor pesticide use in New Zealand. The detection technique used in these surveys is chromatography. The major drawbacks of this standard technique are that it is expensive, time consuming and needs significant operator expertise. As an alternate to chromatography, surface plasmon resonance (SPR) aptasensors based on bimetallic and porous sensor chips are proposed. SPR biosensors are portable, cost-effective, label free, user friendly and allow on-site monitoring. Here, the bimetallic sensor chip consists of silver and gold layers deposited on a glass prism by thermal evaporation. Alternatively, porous silver structures are fabricated using a freeze casting technique. Thiol-terminated aptamers are chosen as the biorecognition element due to the direct chemisorption of thiol on gold surfaces. By developing bimetallic and porous SPR aptasensors, the sensitivity and specificity of conventional gold standard SPR biosensors will be enhanced and economical, in-field, real time pesticide monitoring will be one step closer to reality.